Table 1.

Primers and PCR Conditions

Exon	Primers	AnnealingTemperature(°C)	Amplimer Size(bp)
PR1 (−594/−431)	Forward: 5′-TGCAGCACCCAAGTGGAAATTC-3′	60	229
	Reverse: 5′-TCATGCACCTCCTTTCCTGCAG-3′
PR2 (−510/−215)	Forward: 5′-TGATACTCCTGAGTTTCAT-3′	58	338
	Reverse: 5′-AGCACCGCGAGATATCTAATAGC-3′
PR3 (−261/−55)	Forward: 5′-GTCGCCTAGGCAACAGGCGCG-3′	60	262
	Reverse: 5′-TAGCCGCTGCTCTTCTTCATG-3′
PR4 (−68/+108)	Forward: 5′-GCAGGCGTGATGCGTAGTTC-3′	60	218
	Reverse: 5′-GACTTGTCGATCTGCTCGAA-3
Exon 1	Forward: 5′-CTGGCAGGCGTGATGCGTAG- 3′	68	300
	Reverse: 5′-GATGTTCAAGCGGCCTAAGCG-3′
Exon 2	Forward: 5′-GTCTTCCTCAGTGTCTGACGGTC-3′	68	192
	Reverse: 5′-GCCTGCAGCAAAGGAAGAGC-3′
Exon 3	Forward: 5′-TGTACACATTCAGCTTTTCC-3′	58	226
	Reverse: 5′-TGAGGTGTGGATAACAGC-3′
Exon 4	Forward: 5′-TCGTTAGCTGACTGCAGAAGG-3′	60	244
	Reverse: 5′-ATGGTAACTATATGGAGTGG-3′
Exon 5	Forward: 5′-TCATGAAGCTTGTGGGATGTGC-3′	60	316
	Reverse: 5′-TGACATTAGGTAATATCACC-3′
Exon 6	Forward: 5′-GAAGGTGACTCTGTGTGTAC-3′	64	349
	Reverse: 5′-TCACAGCAGAGCAGGTAGAGGAGC-3′
Exon 7	Forward: 5′-TGAGAATTGGACCTGGC-3′	62	283
	Reverse: 5′-TACAACTGACAGCCTCCTTC-3′
Exon 8	Forward: 5′-TGACAAACCAGCCGAAGTGAAC-3′	62	354
	Reverse: 5′-TCAGTACCAGCGTGTGTTTC-3′
Exon 9	Forward: 5′-GAGTACCCATCTCTGCCCTCTG-3′	62	324
	Reverse: 5′-CAGATGCACCACAGAACTGAATCC-3′
Exon 10a	Forward: 5′-CATGAGGCGACAGCACTGC-3′	62	350
	Reverse: 5′-CTTCCATTCCCAGTACTACCTGA-3′
3′ UTR A	Forward: 5′-AGGACGGTTGTAAGCAGTTGTT-3′	62	329
	Reverse: 5′-GGAACCACAGCCAGTTTATGAT-3′
3′ UTR B	Forward: 5′-TCTCTGCTTACTAATGTGCCCC-3′	60	351
	Reverse: 5′-TCAAGAGTGTCGACTTGGA-3′
3′ UTR C	Forward: 5′-TCTGGACATGATTTAGGGAAGC-3′	62	320
	Reverse: 5′-TGCCGTGTTTTTCATGTTAAAA-3′
3′ UTR D	Forward: 5′-AAAGGGAACTGAAGACCTCCAC-3′	64	314
	Reverse: 5′-GGAGGTTAAACCAGTACGTCCA-3′
3′ UTR E	Forward: 5′-ATTCTGAGCTGGCTTGTTGTTT-3′	62	234
	Reverse: 5′-GGTTTATTCCATGGCCATTGTA-3′
Intron 9 A	Forward: 5′-CTGGTCAACATGTGCGTGG-3′	62	267
	Reverse: 5′-CTTGGGACCAGAGGGCTC-3′
Intron 9 B	Forward: 5′-TTAAGGATCGATGCACTGGG-3′	62	324
	Reverse: 5′-TTGGGATTCCTTCCCTGGG-3′
Intron 9 C	Forward: 5′-CCTTAGGTGATGTAATCAGCC-3′	62	350
	Reverse: 5′-AGGCCTCGAGGACACCC-3′
Intron 9 D	Forward: 5′-CCTGTGACAGCCATCTTGC-3′	62	327
	Reverse: 5′-CATCTGATGTACTCAGATGCC-3′
Intron 9 E	Forward: 5′-ACTAGCCTCAGAGCTCTGG-3′	58	348
	Reverse: 5′-GTGGAGGTGGAGTGGAGG-3′

^aProximal region of the 3′ UTR.