Figure 2.

Immunostaining of phosphorylated IRS-1 in AD brain was variable and partially colocalized with the GVD. A, Immunostaining of phospho-IRS-1 Ser312 showed increased IRS-1 staining (green) typically in tangle-bearing neurons (AT8; red), and this colocalized with AT8. B, C, pIRS-1 Ser312 labeled apparent GVD. pIRS-1 was occasionally colocalized with phosphorylated tau in GVD (arrowheads indicate neurons containing granules). D, No phospho-IRS-1 and tau staining were observed in the absence of primary antibodies. Scale bar, 20 μm.