Figure 4.

Aβ42 oligomers induced aberrant inactivation of IRS-1 Ser616 in cultured hippocampal neurons. Western immunoblot analysis of pIRS-1 induced by Aβ42 oligomers in primary hippocampal neurons. Hippocampal neurons cultured for 9 DIV were treated with 100 nm Aβ oligomers. Harvested cells were then sonicated in lysis buffer and Western blotted with anti-pIRS-1Ser616 antibody. pIRS-1 levels were significantly increased in the membrane fraction after 5 h of Aβ42 oligomer-treatment (17.218 ± 0.922) when compared with controls (7.170 ± 0.674) (p = 0.013). β-Actin was used to normalize protein loading. Shown are mean ± SE. The experiment was repeated three times.