Figure 5.

Aβ42 oligomers induced aberrant activation of JNK-sensitive tau Ser422 in cultured hippocampal neurons. Western immunoblot analysis of ptau induced by Aβ42 oligomers in primary hippocampal neurons. Lysis fractions from 9 DIV hippocampal neurons treated with or without 100 nm Aβ oligomers were Western blotted with an anti-ptau-1Ser422 antibody. ptau levels were significantly increased in the cytosol fraction after 5 h in Aβ42 oligomer-treated neurons (21.161 ± 2.407) when compared with controls (5.488 ± 1.000) (p = 0.004). β-Actin was used for normalization. Shown are mean ± SE. The experiment was repeated three times.