Skip to main content
. 2009 Jul 15;29(28):9078–9089. doi: 10.1523/JNEUROSCI.1071-09.2009

Figure 7.

Figure 7.

DHA suppressed Aβ oligomer-induced aberrant inactivation of IRS-1 Ser616 and JNK-sensitive tau Ser422 phosphorylation in cultured hippocampal neurons. Hippocampal neurons were cultured for 9 DIV and then pretreated either with 2.5 μm DHA for 48 h or with the JNK inhibitor SP600125 (10 μm) for 30 min, followed by 100 nm Aβ42 oligomers for 5 h, after which pIRS-1 and ptau were evaluated by Western blot. A, B, Western immunoblot analysis of pIRS-1 showed induction by Aβ42 oligomers, a response antagonized by either DHA or JNK inhibitor (SP600125). pIRS-1 protein levels were significantly increased in membrane fractions (B) (***p < 0.001) by Aβ42 oligomers, but not if DHA or JNK inhibitor SP600125 was also present (B). A, C, Western immunoblot analysis shows that ptau was induced by Aβ42 oligomers, a response that was blocked by JNK inhibitor SP600125. ptau protein levels were significantly increased in cytosol (C) (***p < 0.001), but not if DHA or JNK inhibitor SP600125 was also present. β-Actin was used for normalization for protein loading. Error bars indicate SEM.