Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 22;10:57. doi: 10.1186/1742-9994-10-57

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Valigurová et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Actin and myosin in Gregarina cuneata gamonts. A. Gamonts in syzygy; primite (p), satellite (s). LM, transmitted light. B. Localisation of F-actin in a gamont; nucleus (asterisk), septum (arrowhead) between protomerite and deutomerite. CLSM, phalloidin-TRITC. C-D. Localisation of F-actin in gamonts (previously associated in syzygy) treated for 10 minutes with 10 μM JAS. Intense labelling is restricted to the cortex and cytoplasmic F-actin aggregations; septum (arrowhead), nucleus (asterisk). Figure C shows a primite. CLSM (left) and merged CLSM/transmitted light (right), phalloidin-TRITC. Figure D shows a satellite. CLSM, phalloidin-TRITC. E. The deutomerite of a gamont treated for 10 minutes with 10 μM JAS. F-actin localisation corresponds to the cortex and nucleus (asterisk). Upper two figures show the gamont middle plane; lower figure shows the cortex in the area of epicytic folds. Merged CLSM/transmitted light (upper) and CLSM, phalloidin-TRITC. F. F-actin localisation in a gamont treated for 150 minutes with 10 μM JAS. Note the decreased labelling of cell cortex and septum (arrowhead), and formation of numerous cytoplasmic aggregations of F-actin; nucleus (asterisk). CLSM, phalloidin-TRITC. G. The deutomerite of a gamont treated for 150 minutes with 10 μM JAS. F-actin labelling is restricted to the cortex in the area of epicytic folds (lower); cytoplasmic F-actin aggregations (upper). CLSM, phalloidin-TRITC. H. Actin localisation in previously associated gamonts; septum (arrowheads). CLSM, IFA. I. Actin localisation in a gamont ghost; nucleus (asterisk), septum (arrowhead). CLSM, IFA. J. Myosin labelling in a maturing gamont. CLSM, IFA. K. Myosin labelling in a mature gamont is restricted to the cortex, but not to the septum (arrowhead). Merged CLSM/transmitted light, IFA. L. Labelling of myosin in a gamont cortex shows a pattern of longitudinal rows; septum (arrowhead). CLSM, IFA. Figures H, I and J show merged FITC (antibody) and rhodamine (counterstaining with Evans blue) fluorescence channels.