Table 3.
Species/Cytochalasin D concentration |
Gregarina cuneata |
Gregarina polymorpha |
Gregarina steini |
||||||
---|---|---|---|---|---|---|---|---|---|
|
10 μM CytD |
20 μM CytD |
30 μM CytD |
10 μM CytD |
20 μM CytD |
30 μM CytD |
10 μM CytD |
20 μM CytD |
30 μM CytD |
Changes/time left after drug application (in minutes) | |||||||||
Initial decrease of gliding speed |
≥ 5 min+ |
≥ 5 min++ |
≥ 5 min+++ |
≥ 5 min+ |
≥ 5 min++ |
≥ 5 min+++ |
≥ 5 min+ |
≥ 5 min++ |
≥ 5 min+++ |
Further decrease of gliding speed |
≥ 20 min+ |
≥ 10 min++ |
≥ 10 min+++ |
≥ 15 min+ |
≥ 10 min++ |
≥ 10 min+++ |
≥ 10 min+ |
≥ 10 min++ |
≥ 10 min+++ |
Cellular changes (shrivelling) |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
≥ 30 min |
Complete stoppage of gliding motility |
≤ 120 min |
≤ 90 min |
≤ 75 min |
≤ 75 min |
≤ 60 min |
≤ 30 min |
≤ 30 min |
≤ 20 min |
≤ 10 min |
Recovery after washing in Ringer’s solution |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
≤ 1 min |
Full recovery after washing in Ringer’s solution | ≥ 5 min | ≤ 10 min | ≤ 10 min | ≥ 5 min | ≤ 10 min | ≤ 10 min | ≥ 5 min | ≥ 10 min | ≥ 10 min |
The symbol + indicates an intensity of observed change ranging from + to +++ when compared between gregarine species and concentrations of cytochalasin D. Applied only if the listed phenomenon showed significant differences in its intensity.