Figure 4.

TANK can regulate the S-phase progression and contributes to cell migration. (a) The indicated cell lines were used to knockdown TANK or TBK1. After selection with puromycin, cells were used for quantitative analysis of cycle phases using a FACSCalibur (upper) and also for western blotting to ensure efficient knockdown (lower). (b) A764 cells were transduced to knock down expression of TANK or TBK1 as shown and then treated for the indicated periods with vinblastine. The cell cycle phases of propidium iodide stained cells were determined by fluorescence-activated cell sorting (FACS) as shown. (c) The indicated knockdown and control U251 cells were grown to density and treated with the DNA polymerase inhibitor aphidicolin (1 μg/ml) to prevent proliferation. After scratching of the cell monolayer migration of cells was examined using a NIKON Eclipse TE2000-E life cell imaging microscope (Nikon, Düsseldorf, Germany). The right part shows a quantitative analysis of cell migration, error bars were derived from two independent experiments performed in triplicates. (d) The experiment was done as in (c) with the exception that U373 cells were analyzed.