Figure 5.

Identification of signaling pathways regulated by the noncanonical IKK complex. (a) The indicated cell lines were used to knock down TBK1 and subsequently analyzed for protein expression and phosphorylation with the indicated antibodies. (b) The TBK1-dependent coregulation between AKT S473 and T308 phosphorylation is schematically displayed, downward arrows show inhibition of phosphorylation. (c) The experiment was done as in (a) with the exception that TANK was knocked down. (d) Schematic display of absent coregulation between AKT and ERK1/2 phosphorylation after downregulation of TANK.