Figure 6.
Prenatal cocaine exposure reduced the surface expression of GABAA receptor subunits in the mPFC. A, Example immunoblots of the lysates of mPFC tissues obtained from P27 rats that were treated prenatally with saline or cocaine, showing the level of biotinylated membrane-bound fraction (M) and of the total protein (T) for GluR1, GABAA receptor subunits α1, β2 and β3, and actin. B, Densitometric measurements of the band densities of immunoblots for membrane-bound and total levels of various receptor subunits in the mPFC. The data are presented as the ratio of densities measured for cocaine- versus saline-treated rats in the same immunoblot (n = 3). *p < 0.05, **p < 0.01, t test. Error bars indicate SEM.