Figure 3.

PRRSV replication characteristics in porcine nasal mucosa explants at different hours post inoculation. (A) Fluorescence microscopical images of nasal mucosa tissues inoculated with PRRSV strains LV and Lena at 72 hpi. PRRSV inoculated explants were immunostained with mouse anti-nucleocapsid and goat anti-mouse IgG_2a (green). Nuclei are visualized with Hoechst staining (blue). EP: epithelium, LP: lamina propria. White lines indicate the border between the lamina propria and the mucosal epithelium. Scale bar = 50 μm. (B) PRRSV-positive cells were counted in 25 sections. Tissues and medium were collected to study viral production. Error bars show ± SD and a Student’s t-test was performed to evaluate significant differences between samples, *denotes a P value ≤ 0.05.