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Double immunofluorescence stainings of nasal mucosa explants. Tissue samples were sectioned (9 μm) and co-immunostained for PRRSV N-protein (red) and markers for (A) CD163 or (B) Sn (green) at 72 hpi. EP: epithelium, LP: lamina propria. White lines indicate the border between the lamina propria and the mucosal epithelium. White arrows show CD163+PRRSV+, Sn+PRRSV+ or Sn-PRRSV+ cells. Scale bar = 50 μm.
