Figure 4.

Verification of RNA-seq results by qRT-PCR. Eighteen unique transcripts with significantly altered expression pattern in response to dehydration were selected from transcription factors, signal components, and biochemical pathways. (A), Comparison of expression level of unique transcripts between RNA-seq and qRT-PCR. Primers for qRT-PCR are listed in Additional file 4. (B), Scatter diagram of log ratios (Log₂ FC) of unique transcripts. qRT-PCR data were normalized using the ‘housekeeping’ gene CmUBI.