Figure 10.

Inhibition of PP2A by OA increases apoptosis in a time-dependent manner. The presence of apoptotic neurons examined by TUNEL–tetramethylrhodamine red staining in 7 DIC cortical neurons. Nuclei were counterstained using DAPI. TUNEL-positive cells were increased during treatment of cortical neurons with OA in a time-dependent manner. A, The neuronal death is increased by nearly 28% (2.5 h), 43% (5 h), and 78% (10%) after treatment with 0.25 μm OA. B, The quantization in the bar graph represents TUNEL-positive counts from four separate experiments, in which 14 independent fields were counted. Ctrl, Control.