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OA-mediated increase in phosphorylated NF is reduced by overexpression of DN Pin1. Five-day-old cortical neurons were transfected with GFP–DN Pin1 and after 24 h were treated with 0.25 μm OA for 2.5 h. Neurons were immunostained, phospho-NF was detected using SMI31 (red), and DN Pin1 was detected through expression of GFP. Note that only neurons that show DN Pin1 expression exhibited significant reduction of phosphorylated NF in the cell body. Scale bar, 20 μm.
