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. Author manuscript; available in PMC: 2014 Apr 14.
Published in final edited form as: Dev Cell. 2013 Oct 14;27(1):10.1016/j.devcel.2013.08.015. doi: 10.1016/j.devcel.2013.08.015

Figure 4. Male Pectoral Fin ET Undergo Vigorous Renewal.

Figure 4

(A) Section images of male dkk1b:EGFP pectoral fin ET at 1 hour, 1 day, 7 days, or 14 days after EdU injection. Arrows indicate EdU+dkk1b:EGFP-expressing cells. Green: EGFP immunofluorescence; red: EdU immunofluorescence; blue: DAPI.

(B) Whole-mount images of TCFsiam; dkk1b:EGFP pectoral fins. Males expressed both reporters in ET.

(C) Section image of a male TCFsiam; dkk1b:EGFP pectoral fin ET. Arrowheads indicate TCFsiam+ cells. Cuticle shows red autofluorescence in these images.

(D) Ubiquitous overexpression of dkk1b decreased the number of ET in male pectoral fins and reduced their definition. Data are shown as mean ± s.d., *P < 0.001 by two-tailed Student’s t-test.

Scale bars = 10 μm (A, C). See also Figure S3.

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