Figure 4.

Identification of small RNAs derived from the 5.8S rRNA of Bombyx mori. (A) Mapping of reads to the 5.8S rRNA. A total of 746 reads with read copy numbers of no less than 50 were mapped mainly to the 5′ and 3′ termini of the 5.8S rRNA. The designed probes also appeared on the corresponding locations. (B) The sequences of the probes used to identify the small RNAs. The p1, p2 and p3 were overlapped on the 5.8S rRNA sequence. (C) The small RNAs derived from 5.8S rRNA were identified by Northern blotting. Their size was approximately 50nt. The small RNAs originating from the other locations on the 5.8S rRNA were not identified. N: normal BmN cells; V: BmNPV-infected BmN cells.