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. Author manuscript; available in PMC: 2013 Dec 4.
Published in final edited form as: J Agric Food Chem. 2011 Oct 4;59(20):10.1021/jf201643s. doi: 10.1021/jf201643s

Table 1.

Gradient Used for Elution of Pigments by HPLC Reverse Phasea

time (min) solvent A solvent B solvent C flow (mL/min) curve
0.00 75.0 25.0 0.0 1.25 1
8.00 25.0 75.0 0.0 1.25 6
10.00 25.0 0.0 75.0 1.25 6
11.00 19.0 0.0 81.0 1.25 6
13.00 14.0 0.0 86.0 1.25 6
15.00 11.0 0.0 89.0 1.25 6
16.00 0.0 0.0 100.0 1.25 6
18.00 0.0 0.0 100.0 1.25 6
20.00 75.0 25.0 0.0 1.25 1
a

(1/1/8) (v/v/v) water/ammonium acetate 1 M/methanol (solvent A), acetonitrile (solvent B), and reagent alcohol (solvent C).