Figure 7.

Treating CD11b–DTR mice with DT reduces the total number of peritoneal and brain macrophages and induces a marked reduction in cytokine production after brain injury. A, Representative flow cytometry plot of cells obtained from peritoneal lavage of injured CD11b–DTR mice treated with DT or vehicle alone after staining for F4/80 (macrophage marker) and Gr-1 (neutrophil marker). There is a reduction in F4/80-positive cells (macrophages), as quantified in B (***p < 0.001). C, Immunofluorescence staining for CD11b and DAPI in injured brains from CD11b–DTR mice (3 d after injury) treated with or without DT. D, Quantitative RT-PCR analysis of injured brains (3 d after injury) from mice treated with DT or vehicle alone (***p < 0.001). Images in C taken at 20× magnification.