Figure 2.

Functional analysis of sfl and ttv mutant synapses. A, Representative traces of evoked neurotransmission at abdominal muscle 6 of wild type (+/+), sfl^9B4/sfl⁰³⁸⁴⁴ mutants, ttv^00681b mutants, and ttv^00681b mutant larvae expressing a UAS-ttv-myc transgene driven by elav-Gal4, G14-Gal4, or da-Gal4. B, Average EJP amplitudes evoked in sfl or ttv heterozygous and mutant animals, and in ttv mutant animals expressing UAS-ttv-myc as in A. In all graphs, error bars represent SEM. **p < 0.001 relative to +/+ and the relevant heterozygous control. Sample numbers (n) shown below the graph also apply to D. Both sfl and ttv mutants display increased EJP amplitudes compared with heterozygous controls. Expression of Ttv-myc with elav-Gal4 or G14-Gal4, but not da-Gal4, rescues the EJP amplitude of ttv mutants. C, Representative traces of spontaneous neurotransmission in abdominal muscle 6 of animals with the same genotypes as in A. mEJP amplitudes are increased in sfl and ttv mutants, and rescued by neuronal (elav-Gal4) or, to some extent, muscle (G14-Gal4) expression of Ttv-myc. D, Average mEJP frequency for the indicated genotypes (as in B). sfl and ttv mutants have decreased mEJP frequencies relative to +/+ and heterozygous controls; this phenotype is rescued by UAS-ttv-myc expression with G14-Gal4 or da-Gal4, but not elav-Gal4 (Table 1).