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. 2013 Sep 28;13:144. doi: 10.1186/1471-2229-13-144

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Copyright © 2013 Trobacher et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Impact of pH and Ca²⁺/calmodulin on the activity of various recombinant apple and Arabidopsis GADs. Each panel (A-C) represents a separate experiment and shows results from recombinant proteins prepared simultaneously. The data represent the mean of three technical replicates for each treatment. All proteins, except those tagged with a 6-His motif (designated with the prefix H), were purified by calmodulin-affinity chromatography. While the activity of MdGAD1 was detectable at pH 7.3, with the scale chosen for presentation, the low activity is not evident in the figure. SDS-PAGE analysis of the expression and purification of these proteins is given in Additional file 2: Figure S2.