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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Aug 5;191(5):10.4049/jimmunol.1203268. doi: 10.4049/jimmunol.1203268

Figure 8. Inhibition on IFN production in IRAK2[E525A] × IRAK1[D359A] mice correlates with impaired activation of IKKβ.

Figure 8

(A) pDCs from WT or IRAK2[E525A] × IRAK1[D359A] mice were not stimulated (NS) or stimulated for 12 h with 0.05 μM of CpG B, 1 μM CpG A or 25 μg/ml of poly(dU) and the concentration of IFN-α in the cell culture medium measured by ELISA. (B) As in (A) but pDCs from WT or IRAK2[E525A] mice were compared. (C) As in (B) except WT pDCs were compared with pDCs from IRAK1[D359A] mice. The experiments were performed in 96 well plates with three wells being used for each condition, each containing pDCs from a different mouse. Bars represent the Mean ± SEM of one representative experiment. (A-C) *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005. Similar results were obtained in two to three independent experiments. (D) pDCs from wild type (WT) or IRAK2[E525A] mice were stimulated for the times indicated with 0.05 μM CpG B and cell lysates subjected to SDS-PAGE followed by immunoblotting with the antibodies indicated. The blot shown is representative of three independent experiments. (E) As in (D) except pDCs from wild type (WT) or IRAK1[D359A] were compared. (F) As in (E) but pDCs cells from WT and IRAK2[E525A] × IRAK1[D359A] mice were compared. The blots shown are representative of two to three independent experiments.