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. 2013 Dec 4;33(49):19276–19283. doi: 10.1523/JNEUROSCI.3487-13.2013

Figure 1.

Figure 1.

LRP1 mediates Aβ uptake and degradation in neuronal cells. A, NT2 cells were transfected with LRP1-siRNA and used for analysis 48 h after transfection. Western blotting showed that LRP1 expression was suppressed by LRP1-siRNA. Cellular uptake of Aβ42 (1 μm) was analyzed by ELISA in control and LRP1-suppressed NT2 cells after incubation for 24 h. B, Subcellular localization of internalized FAM-Aβ42 (1 μm) and its colocalization with lysosomal marker were observed by confocal microscopy in control and LRP1-suppressed NT2 cells after incubation for 24 h. C, Control and LRP1-suppressed NT2 cells were allowed to internalize Aβ42 (1 μm) for 2 h at 37°C (gray bars); parallel cultures were washed and incubated for an additional 8 h in medium lacking Aβ42 (black bars) and analyzed by ELISA. The decrease of internalized Aβ after 8 h of incubation is estimated as cellular clearance. Data were plotted as mean ± SD (n = 3). *p < 0.05; **p < 0.01.