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. Author manuscript; available in PMC: 2013 Dec 4.
Published in final edited form as: Virology. 2013 Jun 12;443(1):28–39. doi: 10.1016/j.virol.2013.04.019

Fig. 2.

Fig. 2

FRET assay of fluorogenic ATPase and short dsDNA. eGFP-gp16 was incubated with Cy3-DNA and without ATP and excited at 480 nm. Energy transfer occurs between the two fluorophores, with light emission at ~560 nm, indicating that gp16 and DNA are in close proximity. Bar graph (top right) showing the FRET efficiency difference between the two samples.