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. 2013 Oct 1;27(19):2086–2098. doi: 10.1101/gad.224899.113

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© 2013 Ruffell et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 4. — CtsC promotes progression of SCCs but not mammary carcinomas. (A) Percentages of ear skin (area) exhibiting hyperplasia by 1 mo of age (Hyp) or dysplasia by 4 and 6 mo of age (Dys). n = 9–29 mice per category. (B) Percentage of SCC-free in HPV16/CtsC^+/− (blue) and HPV16/CtsC^−/− (red) cohorts. (C) Grading of SCC tumors from HPV16/CtsC^+/− (right) and HPV16/CtsC^−/− (left) mice. n = 239 mice (HPV16/CtsC^+/− cohorts); n = 323 mice (HPV16/CtsC^−/− cohorts). (D) Mammary tumor incidence for MMTV-PyMT/CtsC^+/− (blue) and MMTV-PyMT/CtsC^−/− (red) animals shown by the percentage of mice without visible tumors. n = 120 mice per group. (E) Total tumor burden for mice at day 95 and at end stage (day >100). n = 18–23 mice per group. (F) Enzyme activity of chymase was assessed in skin ear tissue lysates from negative littermates (−LM), HPV16/CtsC^+/− (blue) and HPV16/CtsC^−/− (red) cohorts at 1, 4, and 6 mo of age; and SCC tumor tissue. n = 3–4 mice per group, with each sample assayed in triplicate. Significance was determined by an unpaired t-test; (*) P < 0.05; (**) P < 0.01; (***) P < 0.001. (G,H) Percentage of SCC-free (G) and SCC tumor grading (H) in HPV16/mMCP4^−/− cohorts (red) compared with HPV16/mMCP4^+/− control cohorts (blue). n = 64 mice (HPV16/mMCP4^+/− cohorts); n = 66 mice (HPV16/mMCP4^−/− cohorts). (I) Enzyme activity of NE was assessed in skin ear tissue lysates from negative littermates (−LM); HPV16/CtsC^+/− (blue) and HPV16/CtsC^−/− (red) cohorts at 1, 4, and 6 mo of age; and SCC tumor tissue. n = 3 mice per group, with each sample assayed in triplicate. Significance was determined by an unpaired t-test; (*) P < 0.05. (J,K) Percentage of SCC-free (J) and SCC tumor grading (K) in HPV16/NE^−/− cohorts (red) compared with HPV16/NE^+/− control cohorts (blue). n = 53 mice (HPV16/NE^+/− cohorts); n = 94 mice (HPV16/NE^−/− cohorts).