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. 2013 Oct 1;27(19):2125–2138. doi: 10.1101/gad.226951.113

Figure 3.

Figure 3.

SMG5 and SMG7 degrade bound mRNAs. (A–C,E–G) Human HEK293T cells were transfected with a mixture of three plasmids: one expressing β-globin-6xMS2bs mRNA, another expressing MS2-HA or the indicated MS2-HA-tagged proteins, and a third expressing a transfection control containing the β-globin gene fused to the GAPDH 3′ UTR but lacking MS2-binding sites (β-globin-GAP; Control). β-Globin-6xMS2bs mRNA levels were normalized to those of the control mRNA. The normalized values of the β-globin-6xMS2bs mRNA levels were set to 100 in the presence of MS2-HA. Mean values ± standard deviations from three independent experiments are shown in B and F. A and E show Northern blots of representative RNA samples. (C,G) Western blot analysis showing equivalent expression of the MS2-HA-tagged proteins used in the corresponding tethering assays. (D) The domain architecture of SMG7 consists of an N-terminal 14-3-3-like domain, a middle α-helical domain, and a C-terminal PC region.