Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 1;27(19):2139–2146. doi: 10.1101/gad.227454.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Kim et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

Figure 1. — Kinetochore disassembly blocks precocious kinetochore–microtubule interactions. (A) ipl1-as5/ipl1-mn cells (DSY434 and DSY436) were sporulated. 1-NA-PP1 was added (T = 5 h) to allow spindle formation during the pachytene arrest (no β-estradiol added). The distance between CEN1 (CEN1 was tagged with GFP using the lacO/GFP-lacI system) (Straight et al. 1996; Obeso and Dawson 2010) and the closer SPB (Spc42-DsRed) was measured. (A) CEN1 at the pole (≤0.5 μm from the nearest pole). (B) CEN1 distant from the pole (>0.5 μm from the nearest pole) (n = 84). (C,D) ipl1-as5/ipl1-mn cells (DSY428) were sporulated, and 1-NA-PP1 was added (T = 5 h) to allow spindle formation during pachytene arrest (ndt80Δ). Kinetochores (Mtw1-GFP) and SPBs (Spc42-DSRed) were visualized. Examples of a wild-type cell with chromosomes aligned on the metaphase spindle (C) and an ipl1 cell with scattered chromosomes on a precocious spindle (D). In a sample of 44 ipl1 cells (at T = 9 h), 100% had scattered chromosomes. (E–H) ndt80Δ (DSY585) (E,F) and ndt80Δ ipl1-as5/ipl1-mn (DSY588, 1-NA-PP1 at 5 h) (G,H). Samples were prepared for whole-cell immunostaining for kinetochores (Mtw1-myc and Ndc80-GFP) and SPBs (Spc42-DsRed) at meiotic entry (E,G) and prophase (F,H). (E) Clustered Ndc80 was observed in 98% of the cells at meiotic entry (marked by clustered Mtw1; n = 55). (F) Ndc80 was absent in 100% of prophase cells (marked by dispersed Mtw1; n = 31). (G) Clustered Ndc80 was observed in 100% of cells at meiotic entry (marked by clustered Mtw1; n = 51). (H) Ndc80 was absent in 100% of prophase cells (marked by dispersed Mtw1; n = 31). Bar, 2 μm.