Figure 1.

Isolation and characterization of the sel1 mutants. A, Pigment-defective and seedling-lethal phenotypes of the sel1 mutants. The wild-type (ecotypes Columbia and Ws-2) and sel1 mutant alleles were grown on MS medium with 1% (w/v) Suc or soil for 4 and 14 d, respectively. Bar = 1 mm. B, Gene structure of SEL1 (At4g18520). The black box represents an exon, and gray boxes represent the 5′ and 3′ untranslated regions. The positions of the T-DNA insertion in sel1-1, sel1-2 (SAIL_793_F11), and sel1-3 (SALK_054374) are represented by triangles. T-DNA is not drawn to scale. C, Northern-blot analysis of the SEL1 gene in wild-type (WT) and sel1 mutant alleles. Twenty micrograms of total RNA was isolated from 7-d-old seedlings grown on MS medium. 18S rRNA was used as a loading control. D, Molecular complementation of the sel1 mutant by pSEL1::SEL1 genomic DNA. The top row shows the 14-d-old wild type, sel1-2, and the complemented line (Comp.). The bottom row shows northern-blot analysis of SEL1 in the wild type, sel1-2, and the complemented line. 18S rRNA was used as a loading control. [See online article for color version of this figure.]