Figure 2.

The germination phenotype of reil mutants. A, Germination of reil mutants in the cold (10°C) compared with the Col-0 wild type. B, Germination of reil mutants at optimal temperature (20°C) compared with the Col-0 wild type. Significant differences relative to the wild type are indicated within the top section of each graph by circles, squares, diamonds, and triangles (P < 0.001, n = 4–5 plates per genotype). Each plate had approximately 80 seeds. The percentage of germinated seeds with cotyledons (left) and of seeds with a first rosette leaf greater than 1 mm (right) was scored per plate. The total number of germinated seeds per plate (100%) was scored at the last time point of each assay. Note that the rosette leaves of the reil1-1 reil2-1 double mutant emerged later at optimal temperature and did not emerge in the cold (arrow).