Figure 3.

Functionality of the chimeric EFR-BAK1 receptors Etm-B and Btm-E. A, Oxidative burst measurements in N. benthamiana leaves expressing Etm-B, Btm-E, a combination of Etm-B plus Btm-E, or EFR. Leaf pieces were treated with 100 nm elf18 or 100 nm of the inactive analog elf18G5, and ROS production was measured as light units (LU) by a luminometer for 20 min. Data show average and sd for three replicates. Experiments shown are representative for at least five independent repetitions of the experiments with all constructs. B and C, Elf18-induced complexes Etm-B/Btm-E and EFR/BAK1. Coimmunoprecipitation assays with receptor constructs expressed in N. benthamiana leaves. Detergent-solubilized extracts from leaves, treated for 2 min with 100 nm elf18 as indicated, were immunoprecipitated via the GFP tag present at the C terminus of EFR and Btm-E. Western blots were developed with antibodies against the myc tag on BAK1 and Etm-B (top) or the GFP tag present on EFR and Btm-E (bottom).