Figure 3.

Time-course production of ROS in root border-like cells from Arabidopsis after treatment with 1 μm flg22 and staining with fluorescent probes. Observations were made by laser confocal scanning microscopy. Roots were stained with the probe CMH₂DCFDA (A, C, E, and G) to visualize the overall ROS and with the probe SOSG (B, D, F, and H) to specifically visualize the ¹O₂. Fluorescence is indicative of ROS occurrence. Arrows point to border-like cells. eBLC, Elongated border-like cells; R, root; sBLC, spherical border-like cells. Bars = 40 μm. [See online article for color version of this figure.]