Table I. C-related enzyme activities during pre anthesis in flag leaves.

C-related enzyme activities were determined in flag leaves of wild-type and transgenic P_SARK::IPT plants subjected to 3 d of water stress. Enzyme activities are expressed as moles of metabolite generated/consumed per milligram of protein per unit of time. Additional details are provided in “Materials and Methods.” Values are presented as means ± sd of six individual plants per genotype/treatment. The data were analyzed using one-way ANOVA followed by Student’s t test. Boldface values indicate significant differences by genotype comparisons (P ≤ 0.05); asterisks indicate significant differences by treatment comparisons (P ≤ 0.05); CWInv, Cell wall invertase.

Enzyme Activities	Flag Leaf
	Well Watered		Water-Stress
	Wild Type	PSARK::IPT	Wild Type	PSARK::IPT
SPS (µmol Suc mg−1 protein h−1)	24.9 ± 6.5*	18.5 ± 2.7	16.3 ± 3.4	24 ± 2.6*
SuSy (synthesis) [µmol Suc mg−1 protein h−1]	5.9 ± 1.3	5.8 ± 2.6	5 ± 2.4	5.8 ± 2.72
SuSy (degradation) [µmol Fru mg−1 protein h−1]	4.7 ± 0.5	3.3 ± 0.7	5.7 ± 0.4	4. ± 0.8
CytINV (µmol Glc mg−1 protein h−1)	2.9 ± 0.6	2.9 ± 0.2	4.4 ± 0.5	2.3 ± 0.6
VINV (µmol Glc mg−1 protein h−1)	7.7 ± 1.7	9.1 ± 1.2	11.6 ± 1.7*	9.5 ± 0.6
CWInv (µmol Glc mg−1 protein h−1)	9 ± 3.2	11 ± 3.4	11.8 ± 3	9.5 ± 3.9
Amylolytic activity (µmol Glc mg−1 protein h−1)	6 ± 0.8	5.8 ± 0.8	7.9 ± 1.6*	6.7 ± 0.7
Trehalase (µmol Glc mg−1 protein h−1)	0.6 ± 0.1	0.5 ± 0.2	1 ± 0.06*	1.2 ± 0.1*
HK (nmol NADH mg−1 protein min−1)	6.1 ± 0.6	9.4 ± 0.4	8.8 ± 0.9*	12 ± 1.3*