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. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Transpl Immunol. 2013 Oct 9;29(0):10.1016/j.trim.2013.10.002. doi: 10.1016/j.trim.2013.10.002

Fig. 2 — Naïve (Tn) and memory T cells (Tmem) in the peripheral blood (PB) before and after Alemtuzumab infusion. Blood samples were drawn from the heart graft recipient cynomolgus monkeys (n=6) before Alemtuzumab (baseline), on the day of transplant (day 0; 2 days after the first Alemtuzumab infusion), then weekly and monthly thereafter. After gating on CD3⁺CD4⁺ and CD3⁺CD8⁺ cells, Tn (CD95⁻) and Tmem (CD95⁺) subsets were enumerated. (A) Absolute numbers of CD3⁺, CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells (left), and percentages (%) of total CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells (right) in PB. (B) Flow cytometric analysis of Tn (CD95⁻) and Tmem (CD95⁺) in PB at various times, before and after Alemtuzumab infusion. (C) Absolute number and percentage (%) of baseline of CD4⁺ (upper) and CD8⁺ (lower) Tn (CD95⁻) and Tmem (CD95⁺) subsets, before and after Alemtuzumab infusion.

Fig. 2 — Naïve (Tn) and memory T cells (Tmem) in the peripheral blood (PB) before and after Alemtuzumab infusion. Blood samples were drawn from the heart graft recipient cynomolgus monkeys (n=6) before Alemtuzumab (baseline), on the day of transplant (day 0; 2 days after the first Alemtuzumab infusion), then weekly and monthly thereafter. After gating on CD3⁺CD4⁺ and CD3⁺CD8⁺ cells, Tn (CD95⁻) and Tmem (CD95⁺) subsets were enumerated. (A) Absolute numbers of CD3⁺, CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells (left), and percentages (%) of total CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells (right) in PB. (B) Flow cytometric analysis of Tn (CD95⁻) and Tmem (CD95⁺) in PB at various times, before and after Alemtuzumab infusion. (C) Absolute number and percentage (%) of baseline of CD4⁺ (upper) and CD8⁺ (lower) Tn (CD95⁻) and Tmem (CD95⁺) subsets, before and after Alemtuzumab infusion.