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Sequences of real-time PCR primers used to evaluate relative expression of RFamide-related peptide-3 (RFRP-3) and KiSS1 genes in the rat
| Primer | Sequence | Amplicon length (bp) |
|---|---|---|
| KiSS1-F | TGCTGCTTCTCCTCTGTG | 116 |
| KiSS1-R | CCAGGCATTAACGAGTTCC | |
| RFRP-3-F | CTCAGCAGCCAACCTTCC | 165 |
| RFRP-3-R | AAACCAGCCAGTGTCTTG | |
| GAPDH-F | AAGAAGGTGGTGAAGCAGGCATC | 112 |
| GAPDH-R | CGAAGGTGGAAGAGTGGGAGTTG | |
GAPDH;Glyceraldehyde-3-phosphate dehydrogenase.
