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. 2013 Mar 6;7(1):43–48.

Table 1.

Characteristics of the primers used for target genes and internal control


Gene Primer sequences (5′-3′) Annealing temperature (˚C) RT-PCR product size (bp)

GAPDH Forward CCAGGTGGTCTCCTCTGACTTCAAC 58 224
Reverse AGGGTCTCTCTCTTCCTCTTGTGTGCTC
Adipo R1 Forward AAACTGGCAACATCTGGACC 62 288
Reverse GCTGTGGGGAGCAGTAGAAG
Adipo R2 Forward ACAGGCAACATTTGGACACA 62 300
Reverse CCAAGGAACAAAACTTCCCA