Table 1.
Gene | Primer sequences (5′-3′) | Annealing temperature (˚C) | RT-PCR product size (bp) | |
---|---|---|---|---|
GAPDH | Forward | CCAGGTGGTCTCCTCTGACTTCAAC | 58 | 224 |
Reverse | AGGGTCTCTCTCTTCCTCTTGTGTGCTC | |||
Adipo R1 | Forward | AAACTGGCAACATCTGGACC | 62 | 288 |
Reverse | GCTGTGGGGAGCAGTAGAAG | |||
Adipo R2 | Forward | ACAGGCAACATTTGGACACA | 62 | 300 |
Reverse | CCAAGGAACAAAACTTCCCA | |||