Table 2.
Minimal inhibitory concentrations (MICs) of fractions obtained from the solvent hydrolyzable of the methanol extract of Asarum heterotropoides root against harmful and nonpathogenic intestinal bacteria using a microtiter plate-based antibacterial bioassay
| |
|
MIC (mg/mL) |
|||||
|---|---|---|---|---|---|---|---|
|
Gram-positive bacteriab |
Gram-negative bacteriac |
||||||
| Materiala | Cd | Cm | Cp | Sa | Bfd | Ecd | St |
| ME |
14.70 bA |
7.35 cB |
7.35 cB |
14.70 bA |
7.35 bB |
14.70 bA |
7.35 bB |
| HSF |
7.35 cB |
14.70 bA |
7.35 cB |
7.35 cB |
3.67 cC |
7.35 cB |
3.67 cC |
| CSF |
14.70 bA |
14.70 bA |
14.70 bA |
7.35 cB |
7.35 bB |
14.70 bA |
7.35 bB |
| EASF |
7.35 cB |
7.35 cB |
14.70 bA |
7.35 cB |
1.83 dC |
14.70 bA |
7.35 bB |
| BSF |
29.40 aB |
58.80 aA |
29.40 aB |
29.40 aB |
58.80 aA |
58.80 aA |
14.70 aC |
| WSF | NAe | NA | NA | NA | NA | NA | NA |
Means in a column followed by the same lowercase letter and means in a row followed by the same uppercase letter are not significantly different (P > 0.05; Bonferroni test).
aME, methanol extract; HSF, hexane-soluble fraction; CSF, chloroform-soluble faction; EASF, Ethyl acetate-soluble fraction; BSF, butanol-soluble fraction; WSF, water-soluble fraction.
bCd, C. difficile ATCC 9689; Cm, C. paraputiricum ATCC 25780; Cp, C. perfringens ATCC 13124; Sa, S. aureus ATCC 12600.
cBf, B. fragilis ATCC 25285; Ec, E. coli ATCC 11775; St, S. enterica serovar Typhimurium ATCC 13311.
dNonpathogenic bacteria.
eNo activity.