Table 3.
Minimal inhibitory concentrations (MICs) of fractions obtained from the solvent hydrolyzable of the methanol extract of Asarum heterotropoides root against beneficial Gram-positive intestinal bacteria using a microtiter plate-based antibacterial bioassay
| |
MIC (mg/mL) |
||||||
|---|---|---|---|---|---|---|---|
| Materiala | Bbb | Bib | Beb | Blb | Lab | Lcb | Cbc |
| ME |
60.67 aA |
58.50 bB |
58.50 aB |
58.50 aB |
29.30 bC |
58.50 aB |
58.50 aB |
| HSF |
29.30 dB |
29.30 cB |
29.30 bB |
29.30 bB |
29.30 bB |
58.50 aA |
29.30 bB |
| CSF |
55.87 cB |
58.50 bA |
58.50 aA |
29.30 bC |
29.30 bC |
58.50 aA |
58.50 aA |
| EASF |
58.50 bB |
117.0 aA |
58.50 aB |
58.50 aB |
58.50 aB |
55.87 bC |
58.50 aB |
| BSF |
NAd |
NA |
NA |
NA |
NA |
NA |
NA |
| WSF | NA | NA | NA | NA | NA | NA | NA |
Means in a column followed by the same lowercase letter and means in a row followed by the same uppercase letter are not significantly different (P > 0.05; Bonferroni test).
aME, methanol extract; HSF, hexane-soluble fraction; CSF, chloroform-soluble faction; EASF, Ethyl acetate-soluble fraction; BSF, butanol-soluble fraction; WSF, water-soluble fraction.
bLactic acid-producing bacteria: Bb, B. bifidum ATCC 29521; Bi, B. infantis ATCC 25962; Be, B. breve ATCC 15700; Bl, B. longum ATCC 15707; La, L. acidophilius ATCC 4356; Lc, L. casei ATCC 393.
cAcidulating bacteria: Cb, C. butyricum ATCC 25779.
dNo activity.