Table 5.
Minimal inhibitory concentrations (MICs) of Asarum heterotropoides root constituents against seven beneficial Gram-positive intestinal bacteria using a microtiter plate-based antibacterial bioassay
| |
MIC (mg/mL) |
||||||
|---|---|---|---|---|---|---|---|
| Compound | Bba | Bia | Bea | Bla | Laa | Lca | Cbb |
| δ-3-Carene |
12.0 cA |
5.80 cB |
12.0 aA |
12.0 aA |
12.0 bA |
12.0 aA |
0.70 eC |
| Methyleugenol |
23.50 aA |
23.50 aA |
5.80 bC |
1.47 cD |
12.0 bB |
1.47 cD |
23.50 aA |
| 1,8-Cineole |
23.50 aA |
12.0 bB |
2.94 cC |
2.94 bC |
12.0 bB |
2.94 bC |
12.0 bB |
| Pellitorine |
2.94 eB |
5.80 cA |
2.94 cB |
1.47 cC |
2.94 dB |
1.47 cC |
1.47 dC |
| (−)-Asarinin |
5.80 dA |
5.80 cA |
5.80 bA |
1.47 cC |
5.80 cA |
1.47 cC |
2.94 cB |
| α-Asarone |
12.0 cA |
12.0 bA |
5.80 bB |
1.47 cC |
12.0 bA |
1.47 cC |
12.0 bA |
| Safrole |
22.70 bB |
23.50 aA |
12.0 aC |
12.0 aC |
22.70 aB |
12.0 aC |
23.50 aA |
| Ciprofloxacin | 0.031 fB | 0.031 dB | 0.031 dB | 0.063 dA | 0.063 eA | 0.063 dA | 0.031 fB |
Means in a column followed by the same lowercase letter and means in a row followed by the same uppercase letter are not significantly different (P > 0.05; Bonferroni test).
aLactic acid-producing bacteria: Bb, B. bifidum ATCC 29521; Bi, B. infantis ATCC 25962; Be, B. breve ATCC 15700; Bl, B. longum ATCC 15707; La, L. acidophilius ATCC 4356; Lc, L. casei ATCC 393.
bAcidulating bacterium: Cb, C. butyricum ATCC 25779.