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. 2013 Sep 23;12:82. doi: 10.1186/1475-2859-12-82

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Copyright © 2013 Koivistoinen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Glycolic acid production in flask cultivations. Engineered H3675 based Saccharomyces cerevisiae strains were tested for glycolic acid production on different carbon sources in shake flask cultivations on SC media: A)d-glucose (20 g l^-1), B)d-xylose (20 g l^-1) + EtOH (20 g l^-1), C) EtOH. There was no glycolic acid production detected in the parent strain H3675. During the cultivation the pH dropped from pH 5.5 to pH 2.9-3.2. Error bars represent ± the standard error of the mean (SEM; n = 3) and when not visible are smaller than the symbol.