Figure 4.

Effect of REG1 deletion on isocitrate lyase activity in presence of different carbon sources. Isocitrate lyase enzyme activities in the Saccharomyces cerevisiae strain when REG1 is deleted compared to the CEN.PK113-1A based H3675 strain without deletion and to a strain where isocitrate dehydrogenase is deleted and replaced by isocitrate lyase overexpression. SCD = synthetic complete medium with 20 g l^-1d-glucose, SCEG = synthetic complete medium with 20 g l^-1 ethanol and 20 g l^-1 glycerol, SCX = synthetic complete medium with 20 g l^-1d-xylose. Error bars represent ± the standard error of the mean (SEM; n = 3) and where not visible are smaller than the symbol.