Table 1.
Final titers of glycolic acid and biomasses in Saccharomyces cerevisiae flask cultivations
Final titers of glycolic acid (g l-1) | Final biomass (g CDW l-1) | |||||
---|---|---|---|---|---|---|
Strain |
d-glucose |
d-xylose + EtOH |
EtOH |
d-glucose |
d-xylose + EtOH |
EtOH |
H3744 |
0.03 ± 0.02 |
0.1 ± 0.10 |
0.27 ± 0.02 |
5.62 ± 0.04 |
8.08 ± 0.06 |
5.27 ± 0.23 |
H3788 |
0.12 ± 0.00 |
0.51 ± 0.02 |
0.59 ± 0.00 |
3.33 ± 0.06 |
4.65 ± 0.15 |
1.10 ± 0.03 |
H3790 |
0.11 ± 0.01 |
0.55 ± 0.01 |
0.57 ± 0.02 |
3.65 ± 0.06 |
4.43 ± 0.12 |
0.91 ± 0.06 |
H3846 |
0.11 ± 0.02 |
0.90 ± 0.01 |
0.60 ± 0.01 |
3.70 ± 0.15 |
4.98 ± 0.04 |
1.48 ± 0.07 |
H3994 | 0.10 ± 0.01 | 0.91 ± 0.02 | 0.58 ± 0.02 | 3.78 ± 0.06 | 5.35 ± 0.23 | 1.07 ± 0.09 |
Final titers and final biomasses of different glycolic acid producing S. cerevisiae strains grown in SC media containing either d-glucose (20 g l-1), d-xylose (20 g l-1) and ethanol (20 g l-1), or only ethanol (20 g l-1) as carbon sources. The d-xylose utilising Saccharomyces cerevisiae strain H3675 (MATα his3 leu2 trp1 ura3::XYL1-XYL2 xks1::XKS1 MAL2-8c SUC2), a modified [CEN.PK113-1A [50]], was used as a parent strain for all S. cerevisiae glycolic acid production strains. ± presents the standard error of the mean (SEM; n = 3).