Table 3.
Glycolic acid production strains constructed in this work
| Strain number | Modifications | Auxotrophies |
|---|---|---|
| H3744 |
S. cerevisiae H3675 (pGLYR1) |
his3, leu2, trp1 |
| H4241 |
S. cerevisiae H3675 (reg1-Δ1) |
his3, leu2, trp1, ura3 |
| H4242 |
S. cerevisiae H3675 (idp2Δ::ICL1) |
his3, leu2, trp1, ura3 |
| H3788 |
S. cerevisiae H3675 (pGLYR1, mls1-Δ1) |
his3, leu2, trp1 |
| H3790 |
S. cerevisiae H3675 (pGLYR1, mls1-Δ1, dal7-Δ1) |
his3, leu2, trp1 |
| H3846 |
S. cerevisiae H3675 (pGLYR1, mls1-Δ1, dal7-Δ1, idp2-Δ1::ICL1) |
his3, leu2, trp1 |
| H3994 |
S. cerevisiae H3675 (pGLYR1, mls1-Δ1, dal7-Δ1, idp2-Δ1::ICL1, reg1-Δ1) |
his3, leu2, trp1 |
| H3968 |
K. lactis H3954 (mls1-Δ1) |
ura3 leu2 his3 |
| H3976 |
K. lactis H3954 (mls1-Δ1, idp2-Δ1) |
ura3 leu2 his3 |
| H3986 | K. lactis H3954 (pGLYR1, mls1-Δ1, idp2-Δ1) | leu2 his3 |
The xylose utilising Saccharomyces cerevisiae strain H3675 (MATα his3 leu2 trp1 ura3::XYL1-XYL2 xks1::XKS1 MAL2-8c SUC2), a modified VTT C-10880, [CEN.PK113-1A [50]], was used as a parent strain for all S. cerevisiae glycolic acid production strains. The Kluyveromyces lactis strain H3954 [KHO69-8C (MATα ura3 leu2 his3::loxP ku80::loxP)] [51] was used as a parent strain for all K. lactis glycolic acid production strains.