Fig. 9.

(A) Immunohistochemistry of TLR-2 positive cells in lung sections from model D (the left panel) and E (the right panel). Negative control was noted in the lower panel. (B) The percentage of TLR-2 positive AMs in lung sections from mice at 6 days after two immunizations with alum alone (□) or with alum plus MP extracts (■). (C) Relative intensity of TLR-2 mRNA level extracted from AMs in BALF from mice at 6 days after two immunizations with alum alone (□) or with alum plus MP extracts (■). (D) Inhibition assay using MAPK inhibitor, SP600125 for JNK (c-Jun N-terminal kinase) enzymes. The production of IL-6 by AM stimulated with MP extracts (12.5 mg/mL and 50 mg/mL) co-incubated with or without SP600125 (5 μM) in vitro (D).