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. 2011 Nov 11;1(1):76–87. doi: 10.1016/j.rinim.2011.11.001

Table 1.

Experimental schedule.

Events Timing Treatments
Pre-immunization (intraperitoneal) 6 and 13 days prior to intratracheal challenge Pre-immunization with alum alone or with alum plus MP extracts
Intratracheal challenge Day 0 Intratracheal challenge with MP extracts
BAL analysis 0, 8, 24, 48, 96, 168, and 336 h after intratracheal challenge
Sampling for lung pathology 0, 8, 24, 48, 96, 168, and 336 h after intratracheal challenge

alum: a Th2 stimulating adjuvant, MP extract: Mycoplasma pneumoniae extract, derived from centrifugation of sonicated particle of alive Mycoplasma pneumoniae, described in the “Preparation of MP extracts” section above mentioned in the “Material and methods” section.