Figure 4. Tropism of clone derived viruses and their parental isolate.

Ghost-CXCR4 and Ghost-CCR5 cells were treated in the absence or presence of the following inhibitors one hour prior to infection: CXCR4 antagonist AMD3100 at 2.5 µM, or CCR5 antagonist TAK-779 at 20 µM. Approximately 4,000 cells for each sample were collected to perform flow cytometry assay for GFP signals to determine the number of HIV infected cells. The mean numbers of GFP-positive cells are shown with standard deviations from three separate assays.