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. 2013 Nov 18;8(11):e79177. doi: 10.1371/journal.pone.0079177

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© 2013 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The clone-derived virus was produced by transfection into 293T cells and stored at −80°C. Frozen virus supernatant was then thawed to test the TCID₅₀ value on Tzm-bl cells and p24 antigen levels. Virion infectivity was calculated by TCID₅₀/ng p24. The mean values are shown with standard deviations from three separate assays.