Figure 5.

The portal of entry of CyHV-3 in carp analysed by bioluminescence imaging. Two groups of fish (mean weight 10 g) were infected with a recombinant CyHV-3 strain expressing luciferase as a reporter gene either by bathing them in water containing the virus (Immersion, left column) or by feeding them with food pellets contaminated with the virus (Oral, right column). At the indicated time post-infection, six fish per group were analysed by bioluminescence IVIS. Each fish was analysed lying on its right and its left side. To analyze internal signals, fish were euthanized and dissected immediately after in vivo bioluminescence imaging. Dissected fish and isolated organs were analysed for ex vivo bioluminescence. The analysis of one fish is presented for each time point and inoculation mode. Pictures collected over the course of this experiment are presented with a standardized minimum and maximum threshold value for photon flux. rba, right branchial arches; lba, left branchial arches; ro, right operculum; lo, left operculum; p, pharynx; aw, abdominal wall; i, intestine. Reproduced with permission from Fournier et al. [57].