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. 2013 Sep 23;13:234. doi: 10.1186/1472-6882-13-234

Figure 1.

Figure 1

Effect of STE on cell viability and in vitro wound closure in HONE-1 cell. (A) HONE-1 cells were treated with STE (0, 25, 50, 75, 100, 150, and 200 μg/mL) for 24 h and 48 h before a MTT assay for cell viability. The values are means±SD of at least three independent experiments. (B) HONE-1 cells were wounded and then treated with vehicle (DMSO) or STE (0, 25, 50, 75 and 100 μg/mL) in the presence or absence of TPA (50 ng/mL) for 0, 12, and 24 h in 0.5% FBS-containing medium. In the different time points, phase-contrast pictures of the wounds at three different locations were taken. (C) Cells migrating into the wound area were counted using the dashed line as time zero. A quantitative assessment of the mean number of cells in the denuded zone was the mean±SD (n=3). #p<0.05 compared to the vehicle group; *p<0.05 compared with the TPA treatment group.