Table 3.
Substrate | Km (µM) | Vmax, relb | Vmax, rel/Km | n |
---|---|---|---|---|
AMT | 4.4 ± 1.1 | 1.00 | 0.23 | 6 |
13 | 4.7 ± 0.4 | 0.11 ± 0 | 0.023 | 3 |
14 | 1.6 ± 0.2 | 0.74 ± 0 | 0.48 | 2 |
FPGS substrate activity was determined as described in Section 3 at 2 mM l-[3H]glutamate. Values presented are the average ± SD if n ≥ 3 and are average ± range for n = 2.
Vmax, rel is calculated based on the apparent Vmax of a substrate relative to the apparent Vmax of AMT within the same experiment.