. Author manuscript; available in PMC: 2013 Dec 4.

Published in final edited form as: Bioorg Med Chem. 2006 Sep 20;14(24):10.1016/j.bmc.2006.08.029. doi: 10.1016/j.bmc.2006.08.029

Table 3.

Activity of folate analogs as substrates for recombinant human FPGS^a

Substrate	K_m (µM)	V_{max, rel}^b	V_{max, rel}/K_m	n
AMT	4.4 ± 1.1	1.00	0.23	6
13	4.7 ± 0.4	0.11 ± 0	0.023	3
14	1.6 ± 0.2	0.74 ± 0	0.48	2

FPGS substrate activity was determined as described in Section 3 at 2 mM l-[³H]glutamate. Values presented are the average ± SD if n ≥ 3 and are average ± range for n = 2.

V_{max, rel} is calculated based on the apparent V_max of a substrate relative to the apparent V_max of AMT within the same experiment.